On Animals Susan Orlean. Pastoral Song James Rebanks. A Wild Idea Jonathan Franklin. Morphology of Cells in Culture 5.
Morphology of Cells in Culture 6. Be the first to like this. Total views. You just clipped your first slide! Clipping is a handy way to collect important slides you want to go back to later. Now customize the name of a clipboard to store your clips. Visibility Others can see my Clipboard. Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The article introduces three main questions a manufacturer should ask themselves: 1 How do I simplify my process?
Western Blot Analysis is the most widely used technique for protein detection. In this video, we will cover the 3 major steps in generating a western blot: Separate, Transfer, and Detect. The handbook and videos provide an introduction to cell culture, with a focus on maintaining cell health throughout the processes of culturing, freezing, thawing and passaging cells. In this video, we focus on how to passage cells. The new Qubit 4 Fluorometer still has the features you always loved and now comes with a built-in reagent calculator!
This cell culture workflow video focuses on steps pertaining to freezing cells. It also contains useful information, troubleshooting guides and protocols for the experienced researcher as well. Learn about the E-Gel Power Snap System powerful features and how the integrated design combines the convenience of rapid, real-time nucleic acid analysis with high-resolution image capture to reduce workflow time and accelerate discovery.
PCR is a powerful technique in molecular biology. This video covers PCR overview, historical significance, and implications.
In this video, we focus on how to thaw cells. However, not all sera are equal and able to support the proliferation of cells in culture. FBS suffers from high cost, variability, and unwanted biological effects on certain cell types are common hurdles. This is why it is critically important to obtain sera from a reputable supplier. The use of plastic cultureware has become commonplace for cell culture where polystyrene PS is the most frequently used plastic in labs today.
The type of cultureware will depend on whether you are growing adherent or suspension cells as well as the scale of your cell culture, which will dictate the type and size of the culture vessel. PS is hydrophobic in nature making it ideal for suspension cells but many cells are anchorage-dependent adherent cells that require a tissue culture TC —treated surface to promote cell adhesion and spreading.
In this case, a negative charge is imparted to the PS surface by plasma gas which increases its hydrophilicity for cellular attachment. Furthermore, this coating can be optimized for your cell type of interest by coating the TC- surface with extracellular matrix molecules like peptides e. Culture Conditions. The pH, CO 2 and temperature are other physiochemical parameters that require consideration as these requirements are also cell type dependent.
The pH and CO 2 levels are closely linked and are modulated by media components. As cell metabolize, they produce CO 2 , which causes the pH of the culture media to decrease become acidic. Normal cells stop growing when they reach confluence contact inhibition , and it takes them longer to recover when reseeded.
Transformed cells can continue proliferating even after they reach confluence, but they usually deteriorate after about two doublings. Similarly, cells in suspension should be passaged when they are in log-phase growth before they reach confluency. When they reach confluency, cells in suspension clump together and the medium appears turbid when the culture flask is swirled.
While tightly adherent insect cells can be passaged at confluency, which allows for easier detachment from the culture vessel, insect cells that are repeatedly passaged at densities past confluency display decreased doubling times, decreased viabilities, and a decreased ability to attach.
On the other hand, passaging insect cells in adherent culture before they reach confluency requires more mechanical force to dislodge them from the monolayer.
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